High Molecular Weight Ladder

Neb also offers various conventional double stranded dna molecular weight markers with sizes up to 23 kb and markers for pulsed field gel electrophoresis pfge ranging from 1 5 to 1 018 kb.

High molecular weight ladder. Neb dna ladders are available in standard formats and in a variety of convenient ready to load formats. A high molecular weight ladder polymer based on 5 5 6 6 tetrahydroxy 3 3 3 3 tetramethylspirobisindane and 1 4 dicyanotetraflurobenzene has been synthesized by polycondensation under high intensity mixing conditions at about 155 c and cyclic free products were obtained in high yield with low molecular weight distribution 1 7 2 3. The protein standard is supplied in a ready to use format for direct loading onto gels. Compare and view all other protein standards and ladders.

More intense bands on gels and blots and have improved band spacing for more accurate molecular weight determination. The 2 d sds page standards have a pi range from 4 5 to 8 5 and a molecular weight range from 17 5 to 76 kd. High intensity prestained multicolored bands allow distinction of closely related proteins. Therefore when used in gel electrophoresis markers effectively provide a logarithmic scale by which to estimate the size of the other fragments.

A molecular weight size marker also referred to as a protein ladder dna ladder or rna ladder is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. Each for 133 00 add to cart 45 001 592. 6 5 200 kda. Generuler high range dna ladder can be labeled radioactively with t4 polynucleotide kinase see protocol in the resources.

These protein ladders for sds page are available in three size ranges. Low molecular weight range. The ladder is a mixture of chromatography purified individual dna fragments. Protein molecular weight marker ladder.

Each marker is quantitated to yield uniform band intensities when stained with coomassie brilliant blue r 250 following sds page. No need to heat reduce or add sample buffer prior to use. Himark pre stained protein standard is designed for analysis of high molecular weight proteins and consists of nine protein bands ranging from 30 460 kda. Protein dna and rna marker.

14 3 97 2 kda high molecular weight range. Bio rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including sds page ief 2 d page and western blotting. 44 3 200 kda and broad molecular weight range.